Johnston Associates (JAI) is a United States-based venture capital firm founded in 1968 and headquartered in Princeton, New Jersey. The firm focuses on providing seed-stage capital to healthcare-related startups, with a particular emphasis on pharmaceutical and biotechnology companies. It invests its own private capital rather than managing external funds, allowing for flexible investment horizons and structures. JAI has supported the development of companies across areas such as diagnostics, drug discovery, genetic engineering, and medical technologies. In addition to capital, the firm provides managerial, technical, and strategic support to help early-stage companies grow.